PurposeThe effects of high voltage pulsed electric field on the antioxidant activity of tea polysaccharides and polyphenols in Pu'er ripe tea were studied in order to provide a promising raw material treatment method for improving the antioxidant activity of Pu'er ripe tea in vitro.
MethodWe used different high voltage pulsed electric fields (HPEF) to deal with the third grade Pu'er ripe tea, extract the tea polysaccharides and tea polyphenols and determine antioxidant activity of tea polysaccharides and tea polyphenols in each tea sample by ABTS radical scavenging rate. Then antioxidant activity of tea polysaccharides and tea polyphenols were analyzed by stepwise regression analysis on Matlab, we established the mathematical model of its antioxidant activity and voltage, frequency, and drew the three-dimensional relationship diagram of the model to study the effect of different HPEF on the antioxidant activity.
ResultsThe most important HPEF parameter affecting the scavenging ability of tea polysaccharides on ABTS was voltage, and when the HPEF voltage was 12 kV and the frequency was about 120 Hz, the scavenging effect of tea polysaccharide on ABTS radical was the strongest. The most important HPEF parameter affecting the scavenging ability of tea polyphenols on ABTS was frequency, and when the frequency was about 140 Hz, the scavenging effect of tea polyphenols on ABTS radical was the strongest.
ConclusionThe suitable conditions of HPEF can improve the antioxidant activity of tea polysaccharides and tea polyphenols in Pu'er tea to a certain extent. This study provides a reference for the development of natural antioxidants in Pu'er tea.
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