Effects of Feeding Frequency and Level on the Digestive Enzymes and Fat Metabolism Enzyme in the Hepatopancreas of Juvenile Leiocassis longirostris

PurposeThe effects of feeding frequency (2 times/day, 3 times/day) and feeding level (the ratio of daily feeding to experimental fish body weight, 2%, 4%, 6%, 8% and 10%, respectively) on digestive enzymes and hepatic pancreatic fat metabolism enzyme activities of juveniles Leiocassis longirostris were studied, which provided a healthier breeding mode for artificial breeding of L. longirostris.

MethodTwo-way analysis of variance was performed using SPSS 17.0 software and Duncan multiple comparison test was used. The significant level was 0.05.

Result(1) The activities of intestinal protease, intestinal amylase and trypsin of juveniles with the 3 times/day feeding frequency and 6% feeding level were the highest, which was significantly higher than those of the treatment with feeding frequency twice a day and 10% feeding level, the treatments with feeding frequency 3 times/day combined feeding level 2% and 10%, respectively (P<0.05). (2) The activities of fat metabolism enzymes hepatopancreas of carnitine acyl transferase (CACT), acetyl coenzyme A carboxylase (the ACC), lipoprotein ester enzyme (LPL) and hepatic lipase (HL) with the 3 times/day feeding frequency and 6% feeding level were the highest, which was significantly higher than those of the treatment with feeding frequency twice a day and 2% feeding level, the treatments with feeding frequency 3 times/day combined feeding level 8% and 10%, respectively (P<0.05).

ConclusionThe most suitable feeding frequency and level are 3 times/d and 6%, respectively, which is more suitable for the healthy breeding juveniles L. longirostris.