Effects of ABA on Soda Saline-alkali Osmotic Regulation and Oxidative Damage of Alfalfa Seedlings

Bo LI; Huajun LIU; Hao LIN; Hong LI; Zhao YANG

doi:10.12101/j.issn.1004-390X(n).201811077

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2019 > 34(4): 689-694. > DOI: 10.12101/j.issn.1004-390X(n).201811077

Bo LI, Huajun LIU, Hao LIN, et al. Effects of ABA on Soda Saline-alkali Osmotic Regulation and Oxidative Damage of Alfalfa Seedlings[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(4): 689-694. DOI: 10.12101/j.issn.1004-390X(n).201811077

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Effects of ABA on Soda Saline-alkali Osmotic Regulation and Oxidative Damage of Alfalfa Seedlings

1.
College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar 161006, China
2.
Institute of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161005, China

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Received Date: November 24, 2018
Revised Date: June 09, 2019
Available Online: July 22, 2019
Published Date: June 30, 2019

Graphical Abstract

Abstract

Abstract

Purpose To explore the effects of different concentrations of ABA on the physiological effects of alfalfa seedlings under soda saline-alkali stress.

Method The seedlings cultured with WL343HQ alfalfa seeds were treated with 150 mmol/L soda saline-alkali stress and ABA alleviation at different concentrations. The physiological and biochemical changes of leaves of seedlings alleviated by ABA were determined. The alleviation effect of ABA was comprehensively analyzed by membership function method.

Result After spraying ABA on alfalfa seedlings under saline-alkali stress, the contents of soluble sugar, betaine, proline, soluble protein and the activities of POD, PAL, CAT and AXP in alfalfa leaves increased firstly and then decreased with the increase of ABA concentration. The four osmotic regulating substances and four antioxidant enzymes activities reached their maximum at ABA concentration of 50 or 100 μmol/L.

Conclusion According to the comprehensive analysis of membership function value, it could alleviate the soda saline-alkali stress when the concentration of ABA was 25-100 μmol/L. But when ABA concentration was 50-75 μmol/L it has the best mitigation effect on soda salt damage.
- alfalfa,
- abscisic acid,
- soda saline-alkali stress,
- osmotic adjustment substance,
- antioxidant enzymes,
- membership function

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References

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Effects of ABA on Soda Saline-alkali Osmotic Regulation and Oxidative Damage of Alfalfa Seedlings

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