Huan LUO, Guogeng JIA, Zhengxing SONG, et al. Isolation and Identification of Pathogenic Alternariafrom Three Senecioneae Plants[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY(Natural Science), 2018, 33(6): 1046-1053. DOI: 10.12101/j.issn.1004-390X(n).201807015
Citation: Huan LUO, Guogeng JIA, Zhengxing SONG, et al. Isolation and Identification of Pathogenic Alternariafrom Three Senecioneae Plants[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY(Natural Science), 2018, 33(6): 1046-1053. DOI: 10.12101/j.issn.1004-390X(n).201807015

Isolation and Identification of Pathogenic Alternariafrom Three Senecioneae Plants

  • Purpose In order to investigate the damage caused by Alternaria species on Compositae plants.
    Method During 2016—2017, 4 samples from 3 plants of Pericallis hybrida, Senecio cineraria, Gynura divaricateshowing leaf spot or blight symptoms were collected from Hubei, Beijing, Anhui and Yunnan Province, China. Morphological similar large-spored Alternaria strains were obtained by single spore isolation from each sample. Then one representative strain from each host was selected to determine the pathogenicity, morphology and sequence analysis using six regions (rDNA-ITS, GAPDH, Alt a 1, EF1-α, RPB2 and ATPase).
    Result The 4 strains were all causal pathogens on their hosts and identified as Alternaria cinerariae.
    Conclusion This is a new record of Alternaria species in China and it is also the first report causing leaf spot disease on S. cineraria and G. divaricate.
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