Cloning, mRNA Expression and Protein Bioinformatics Analysis of the Expressed Sequence 37 (TEX37) of Porcine Testis

PurposeThe aim of this study was to obtain the properties and function of testis expression sequence 37 (TEX37) gene in Banna mini-pig inbred line (BMI) for further study of the gene in spermatogenesis role.

MethodThe specific primers were designed by using Oligo7 and TEX37 complete coding sequence was amplified from BMI. The TEX37 expression profiles of mRNA in 15 tissues of BMI was detected by real-time quantitative PCR. TEX37 protein sequence was conducted functional analysis and established phylogenetic tree.

ResultWe obtained a 561 bp coding sequence of BMI TEX37 gene with GenBank accession number of KU705619, which encodes a protein of 186 amino acids with GenBank accession number AOC89037. real-time quantitative PCR in multi-tissues indicated that TEX37 gene has the highest expressed in the BMI testis tissue and low expression in the other tissues. Further functional bioinformatics analysis revealed TEX37 protein contained a conserved domains TSC21 without transmembrane helix and signal peptide. Its secondary structure is predominantly random coils and its N-terminus hydrophobic and C-terminal hydrophilic. The TEX37 protein has two kinds of phosphorylation sites. Phylogenetic analysis indicated that BMI had the over 67% genetic similarity with other species.

ConclusionThis study will accumulate some basic data for further research of the TEX37 gene function in pig spermatogenesis.