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Jiaqi WU, Ye XU, Yongzhong LI, et al. Proliferative Rule of Hippeastrum chlorotic ringspotvirus in Taeniothrips eucharii[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2020, 35(1): 15-19, 61. DOI: 10.12101/j.issn.1004-390X(n).201805028
Citation: Jiaqi WU, Ye XU, Yongzhong LI, et al. Proliferative Rule of Hippeastrum chlorotic ringspotvirus in Taeniothrips eucharii[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2020, 35(1): 15-19, 61. DOI: 10.12101/j.issn.1004-390X(n).201805028
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Proliferative Rule of Hippeastrum chlorotic ringspotvirus in Taeniothrips eucharii

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    • Abstract
  • Purpose Hippeastrum chlorotic ringspot virus (HCRV) can cause serious damage and is transmited by Taeniothrips eucharii. The aim of this study was to provide the theoretical evidence for controlling HCRV.
    Method In this study, SYBR Green method of real-time fluorescent quantitative PCR (qRT-PCR) was used, combined with the specific primers for HCRV N gene and β-actin gene of T. eucharii for quantitative analysis of HCRV content in different treatments.
    Reault This method could accurately and specifically quantify the HCRV virus in T. eucharii. quantitative of HCRV RNA. The concentrate of HCRV was the lowest in 1st larvae. There was no statistical difference in 1st larva, 2nd larva and pura. And the concentrate of HCRV was the highest in adults. HCRV was growing gradually after the 1st larvae acquire HCRV.
    Conclusion This indicated that T. eucharii transmitted HCRV as a persistent-propagative manner.
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