Optimization of the Fermentation Conditions of Actinomycete Strain 1331 and Its Effects on Meloidogyne incognita

PurposeIn order to study the optimization of fermentation conditions and explore toxicity effects of 1331 fermented fluid to the second-stage juveniles of Meloidogyne incognita (Mi J2).

MethodSingle-factor and orthogonal test were used in shaking flasks to optimize the fermentation conditions of strain 1331.

ResultThe optimum fermentation culture medium was fermentation mediumⅡ. And the optimal fermentation condition was as follows: medium volume 80 mL in 500 mL shaking flask, inoculation size of 5% (volume fraction), initial pH 7.0, culture temperature 25 ℃, shaking at 150 r/min for 7 days. Under this condition, the sporulation of strain 1331 was 1.68×10⁹ CFU/mL; compared with the control, the hatching rate of Mi J2 was lowered down by 70.59% by dealing with 5% 1331 supernatant fermented fluid 9 d, and the corrected mortality rate of Mi J2 was 88.97% and 100% by dealing with 10% and 20% 1331 supernatant fermented fluid for 48 h.

ConclusionThe strain 1331 fermented fluid had toxicity to Mi J2.