Expression of <i>TNC</i> Gene in Development of Bovine Skeletal Muscle and Differentiation of Preadipocytes

Yanxia QI; Yuqin WANG; Xiaohui ZHANG; Pei LIU; Kunju LIU

doi:10.12101/j.issn.1004-390X(n).201804027

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2019 > 34(2): 241-246. > DOI: 10.12101/j.issn.1004-390X(n).201804027

Yanxia QI, Yuqin WANG, Xiaohui ZHANG, et al. Expression of TNC Gene in Development of Bovine Skeletal Muscle and Differentiation of Preadipocytes[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(2): 241-246. DOI: 10.12101/j.issn.1004-390X(n).201804027

Citation:

PDF (649 KB)

Expression of TNC Gene in Development of Bovine Skeletal Muscle and Differentiation of Preadipocytes

1.
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China
2.
Mutton Sheep Breeding Engineering Technology Research Center of Henan Province, Luoyang 471003, China

More Information

Received Date: April 13, 2018
Revised Date: October 16, 2018
Available Online: January 20, 2019
Published Date: February 28, 2019

Graphical Abstract

Abstract

Abstract

PurposeIn order to analyze the expression pattern of TNC gene during the development of longissimus dorsi muscle and the differentiation of preadipocyte, and the relationship between TNC gene expression level with fat deposition in beef cattle.

MethodsThe expression of TNC gene was analyzed by qRT-PCR technology in longissimus dorsi muscle tissues of 3, 6, 12, 18, 24 and 30 months old Nanyang cattle. The fat content in longissimus dorsi muscle of 24 cattle was determined by Soxhlet extraction. The preadipocyte of cattle was separated and induced differentiation with normal medium or medium with 2 μmol/L limonin, and the expression of TNC gene was analyzed by qRT-PCR technology during the differentiation of preadipocyte in control and treatment groups respectively.

ResultsThe expression of the TNC gene increased gradually in longissimus dorsi muscle after 18 month old in cattle. The muscle tissues were divided into high intramuscular fat group and low intramuscular fat group according to the fat content, and the expression levels showed no significant difference between these two groups. The expression level of TNC gene increased sharply at the 6^th hour of the differentiation, then decreased slowly, but increased again at the end of differentiation with the deposition of lipid droplet in cytoplasm. The differentiation of preadipocytes and lipid accumulation in the cytoplasm could be inhibited by limonin in medium, which also induced a significant decrease of TNC gene expression.

ConclusionTNC gene is involved in the regulation of differentiation and lipid deposition in cultivated preadipocytes and fat tissues, and the up-regulated expression of TNC gene would benefit the lipid accumulation in cytoplasm.
- TNC,
- muscle development,
- adipocyte,
- gene expression

FullText(HTML)

References (22)

References

[1]	FRY C S, KIRBY T J, KOSMAC E, et al. Myogenic progenitor cells control extracellular matrix production by fibroblasts during skeletal muscle hypertrophy[J]. Cell Stem Cell, 2017, 5, 20(1): 56. DOI: 10.1016/j.stem.2016.09.010.
[2]	CÔTÉ J A, GUÉNARD F, LESSARD J, et al. Temporal changes in gene expression profile during mature adipocyte dedifferentiation[J]. International Journal of Genomics, 2017, 2017: 5149362. DOI: 10.1155/2017/5149362.
[3]	RAJPRASAD L, BRENDA J R, CHRISTOPHER M S, et al. Extracellular matrix motion and early morphogenesis[J]. Development, 2016, 15, 143(12): 2056. DOI: 10.1242/dev.127886.
[4]	ADAMS J C, CHIQUET-EHRISMANN R, TUCKER R P. The evolution of tenascins and fibronectins[J]. Cell Adhesion & Migration, 2014, 30: 1. DOI: 10.4161/19336918.2014.970030.
[5]	MURPHY-ULLRICH J E, SAGE E H. Revisiting the matricellular concept[J]. Matrix Biology, 2014, 37: 1. DOI: 10.1016/j.matbio.2014.07.005.
[6]	ERICKSON H P. Tenascin-C, tenascin-R and tenascin-X: a family of talented proteins in search of functions[J]. Current Opinion in Cell Biology, 1993, 5: 869.
[7]	GHERZI R, CARNEMOLLA B, SIRI A, et al. Human tenascin gene. Structure of the 5’-region, identification, and characterization of the transcription regulatory sequences[J]. Journal of Biological Chemistry, 1995, 270: 3429.
[8]	祁艳霞, 张小辉, 王玉琴, 等. 牛肌肉组织中胶原和ADAMTS2基因表达与肉质的相关性分析[J]. 云南农业大学学报(自然科学), 2014, 29(2): 173. DOI: 10.3969/j.issn.1004-390X(n).2014.02.005
[9]	祁艳霞, 张小辉, 王玉琴, 等. 基质金属蛋白酶2, 3, 13基因表达水平与牛肉质性状的相关性分析[J]. 云南农业大学学报(自然科学), 2016, 31(1): 62. DOI: 10.16211/j.issn.1004-390X(n).2016.01.010
[10]	徐铁山, 张小辉, 刘佩, 等. 柠檬苦素对牛前体脂肪细胞分化过程中核纤层蛋白基因表达的影响[J]. 黑龙江畜牧兽医, 2017(3): 10. DOI: 10.13881/j.cnki.hljxmsy.2017.0195
[11]	KABLAN A, SAUNDERS R A, SZKUDLAREK-MIKHO M, et al. Prieurianin causes weight loss in diet-induced obese mice and inhibits adipogenesis in cultured preadipocytes[J]. Journal of Diabetes & Metabolism, 2010, 1(101): 1.
[12]	曲桂娟, 秦贵信. 肉牛肌内脂肪的研究进展[J]. 中国畜牧兽医, 2010, 37(6): 221
[13]	TUCKER R P, CHIQUET-EHRISMANN R. The regulation of tenascin expression by tissue microenvironments[J]. Biochimica et Biophysica Acta-Molecular Basis of Disease, 2009, 1793: 888. DOI: 10.1016/j.bbamcr.2008.12.012.
[14]	IMANAKA-YOSHIDA K, AOKI H. Tenascin-C and mechanotransduction in the development and diseases of cardiovascular system[J]. Frontiers in Physiology, 2014, 5: 283. DOI: 10.3389/fphys.2014.00283.
[15]	BOHANNON-STEWART A, GARY K, BONIFACE K, et al. Expression of potential regulatory genes in abdominal adipose tissue of broiler chickens during early development[J]. Genetics Research International, 2014: 318304. DOI: 10.1155/2014/318304.
[16]	ZHANG X H, QI Y X, GAO X, et al. Expression of ADAMTS4 and ADAMTS5 in longissimus dorsi muscle related to meat tenderness in Nanyang cattle[J]. Genetics and Molecular Research, 2013, 12(4): 4639. DOI: 10.4238/2013.October.18.2.
[17]	QI Y X, ZHANG X H, WANG Y Q, et al. Expression of MMP-1, -2, and -8 in longissimus dorsi muscle and their relationship with meat quality traits in cattle[J]. Genetics and Molecular Research, 2016, 15(1): 1. DOI: 10.4238/gmr.15017593.
[18]	MARIMAN E C M, WANG P. Adipocyte extracellular matrix composition, dynamics and role in obesity[J]. Cellular and Molecular Life Sciences, 2010, 67: 1277. DOI: 10.1007/s00018-010-0263-4.
[19]	CHIOVARO F, CHIQUET-EHRISMANN R, CHIQUET M. Transcriptional regulation of tenascin genes[J]. Cell Adhesion & Migration, 2015, 9(1/2): 34. DOI: 10.1080/19336918.2015.1008333.
[20]	THOH M, BABAJAN B, RAGHAVENDRA P B, et al. Azadirachtin interacts with retinoic acid receptors and inhibits retinoic acid−mediated biological responses[J]. Journal of Biological Chemistry, 2011, 286(6): 4690. DOI: 10.1074/jbc.M110.169334.
[21]	CHUNG C Y, MURPHY-ULLRICH J E, ERICKSON H P. Mitogenesis, cell migration, and loss of focal adhesions induced by tenascin-C interacting with its cell surface receptor, annexin Ⅱ[J]. Molecular Biology of the Cell, 1996, 7: 883.
[22]	KIM S M, MATTHIAS C, RICHARD P T, et al. Tenascin-C at a glance[J]. Journal of Cell Science, 2016, 129(23): 4321. DOI: 10.1242/jcs.190546.

Cited By

Cited by

Periodical cited type(2)

1.	鲍麒，梁春年，郭宪，李吉叶，褚敏，阎萍. 全基因组选择信号解析野牦牛和天祝白牦牛的遗传差异. 基因组学与应用生物学. 2022(04): 731-741 .
2.	张小辉，庞有志，雷莹，赵淑娟，祁艳霞，郭灿甫，刘坤举，刘佩. 鹌鹑性别的分子鉴定方法研究. 中国家禽. 2020(05): 20-23 .

Other cited types(2)

Get Citation

PDF

XML

Article views (3017) PDF downloads (36) Cited by(4)

Turn off MathJax

Article Contents

Abstract

References

Expression of TNC Gene in Development of Bovine Skeletal Muscle and Differentiation of Preadipocytes

Abstract

References

Cited by

Periodical cited type(2)

Other cited types(2)

Catalog

Export File

Citation

Format

Content