Effect of Interfering with E2F7 on the Proliferation of C2C12 Myoblasts

PurposeTo explore the biological role of E2F7 in the development of skeletal muscle cells.

MethodC2C12 myoblasts were used as the research object. Small interfering RNA was designed to interfere with the expression of E2F7 in C2C12 myoblast. C2C12 myoblasts were divided into interfering group and control group. The effect of E2F7 on the proliferation of C2C12 myoblasts was detected by RT-PCR, CCK-8 assay and EdU assay, respectively.

ResultThe expression of E2F7 was significantly higher in C2C12 myoblasts during the proliferative phase than that in the differentiation phase (P<0.01); the viability and ratio of neoplastic cells of C2C12 myoblasts after E2F7 interference were extremely significantly higher than those of the control group (P<0.01). The expression level of Cyclin E was significantly increased (P<0.05), and the expression of Cyclin D and CDK4 was extremely significantly elevated when C2C12 myoblasts interfered with E2F7 (P<0.01). Further finding showed that interference with E2F7 significantly promoted E2F2 (P<0.05) and extremely significantly promoted the expression of E2F1 and E2F3 (P<0.01). At the same time, it extremely significantly promoted the expression levels of microRNAs (miR-7, miR-25, miR-27, and miR-92a) which associated with myoblasts proliferation (P<0.01).

ConclusionThe results suggest that interference with can promote the proliferation of C2C12 myoblasts, and E2F7 may plays a role through regulating the classical E2Fs signaling pathway and myoblasts proliferation-related microRNAs.