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Te PU, Yunhai YANG, Kaibo WANG, et al. Determination of the Nicotinamide Compounds by Using MTT Colorimetric Assay against Pathogenic Bacteria[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(1): 15-21. DOI: 10.12101/j.issn.1004-390X(n).201803032
Citation: Te PU, Yunhai YANG, Kaibo WANG, et al. Determination of the Nicotinamide Compounds by Using MTT Colorimetric Assay against Pathogenic Bacteria[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(1): 15-21. DOI: 10.12101/j.issn.1004-390X(n).201803032
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Determination of the Nicotinamide Compounds by Using MTT Colorimetric Assay against Pathogenic Bacteria

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    • Abstract
  • Purpose To investigate the possibility of antibacterial activity of 7 synthetic nicotinamide compounds against 3 kinds of pathogenic bacteria by MTT assay.
    Methods We evaluated bactericidal activity of 7 synthetic nicotinamide compounds against Xanthomonas vesicatoria, Ralstonia solanacearum and Pseudomonas syringae by MTT assay and plate diffusion method. MTT assay performed to identify bactericidal activities of N-(3-(trifluoromethyl) phenyl) nicotinamide.
    Result The EC50 of N-(3-(trifluoromethyl) pheny) nicotinamide against X. vesicatoria, R. solanacearum and P. syringae by MTT assay were 3.87, 2.05 and 10.33 mg/mL.
    Conclusion The results indicated that MTT assay was more effective than plate diffusion method in evaluating bactericidal activity, and has higher value on application.
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