Effect of Root Applying Proline on the Growth of Wheat Seedling under Chrome Stress

Puepose The effect of proline on chrome (Cr³⁺) toxicity in wheat (Triticum aestivum L.) was studied.

Method Wheat seedlings exposed to 160 mg/L Cr³⁺ were treated with 30 mg/L proline.

Results Cr³⁺ exposure depressed wheat growth. Compared with the single Cr³⁺ stress, after proline application, the root length, plant height, fresh weight of stem leaf, dry weight of stem leaf, root fresh weight and root dry weight of the seedlings increased significantly by 49.4%, 17.9%, 21.7%, 52.0% and 38.7%, respectively. And the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate perxoidase (APX), increased by 8.51%, 4.50%, 8.19% and 9.74%, respectively; the contents of superoxide anion (O₂⁻·), hydrogen peroxide (H₂O₂), and malondialdehyde (MDA), rise significantly by 60.32%, 24.56% and 34.20%, respectively. Ultimately, the root activity increased by 1.67 times.

Conclusion Therefore, exogenous proline could significantly improve the antioxidant capacity of wheat roots, and then it could enhance the resistance ability of wheat and alleviate the toxic effect of chrome on wheat seedlings.