Purpose The effects of No.37, 117 and 120 endophytic fungi strains isolated from Dendrobium nobile on the growth and physiological characteristics of D. nobile under nutritional deficiency condition were detected in this study.
Method The endophytic fungi strains No. 37, 117, 120 and distilled water were sprayed on the root of tissue culture regenerated seedlings of D. nobile. Each group was cultivated for 30 days with 1, 1/2, 1/4 and 1/8 of Hoagland nutrient solution, respectively. After 30 days, the effect of endophytic fungi inoculation was determined by measuring the plant growth and physiological characteristics.
Results With the decrease of Hoagland solution concentration, the endophyte treatment groups were significantly higher than the control group (treated with distilled water) in dry weight, chlorophyll SPAD value, total alkaloids, water-soluble polysaccharides and NPK content. The root length and root shoot ratio increased less than the control group. The changes of various indexes were not completely the same between the three endophytic fungi strain treatments. Strain No.37 treatment showed the most significant ability in dry weight and total alkaloid content increase. Strain No.120 was more prominent in controlling root length and root shoot ratio, promoting water-soluble polysaccharide content and phosphorus and potassium absorption. Strain No.117 showed the most obvious effect on increasing chlorophyll SPAD value and promoting nitrogen absorption.
Conclusion All of these three endophytic fungi strains can promote the growth of D. nobile seedlings under nutrient deficit condition, but they showed different types of effect. Therefore, the combination use of these three endophytic fungi will produce better results.
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