Analysis of Grey Relational Degree of Amino Acid Balance between Protein Feed Raw Materials and Hirudinaria manillensis

Purpose We aimed to study the amino acid balance of the Hirudinaria manillensis and protein feed raw material, in order to provide reference for development of amino acid balanced feed for the leech of the H. manillensis.

Method The high performance liquid column derivative method was used to determine the content of amino acid in the H. manillensis and 11 protein feedstuffs, in order to evaluate gray correlation degree of amino acids balance between 11 kinds of protein feed raw materials and H. manillensis.

Result The grey correlation degree of amino acids balance between 11 kinds of protein feed raw material and H. manillensis from high to low was: beef powder>soybean protein concentrate>plasma protein powder>(pork powder=chicken powder)>(fishmeal>soy protein isolate)>blood meal>hemoglobin>soybean meal>whole milk powder.

Conclusion The grey correlation degree was above 0.8 for seven kinds of protein feed: beef powder, soybean protein concentrate, plasma protein powder, pork powder, chicken powder, fish meal and soy protein isolate, which can be optimized as the protein feed raw material of H. manillensis feed.