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Yang YANG, Shuilian HE, Mingting TANG, et al. Optimization of Preparation Technology of 4-[(α-L-Rhamnosyloxy)Benzyl] Isothiocyanate from Moringa Oleifera Seeds[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(2): 303-308. DOI: 10.12101/j.issn.1004-390X(n).201711027
Citation: Yang YANG, Shuilian HE, Mingting TANG, et al. Optimization of Preparation Technology of 4-[(α-L-Rhamnosyloxy)Benzyl] Isothiocyanate from Moringa Oleifera Seeds[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(2): 303-308. DOI: 10.12101/j.issn.1004-390X(n).201711027
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Optimization of Preparation Technology of 4-(α-L-Rhamnosyloxy)Benzyl Isothiocyanate from Moringa Oleifera Seeds

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    • Abstract
  • PurposeTo optimize of enzymatic hydrolysis and purification conditions of 4-(α-L-rhamnosylbenzyl) isothiocyanate (GMG-ITC) from Moringa oleifera seeds.
    MethodsThrough single factor and orthogonal experiments, we optimized the conditions for enzymatic extraction of glucosinolates from the de-oiled M. oleifera seeds. Then, the high purity GMG-ITC by extraction and recrystallization with methylene chloride were obtained.
    ResultAn optimum condition for enzymatic hydrolysis was obtained as following: material to solvent ratio 1∶60 (g∶mL), temperature 30 ℃, reaction time 9 h, pH 5, by HPLC analyzing.
    ConclusionWe described a simple and efficient four-step process including de-oiling, enzymatic hydrolysis, extraction and recrystallization to afford GMG-ITC from M. oleifera seeds, an overall yield of 4.70% was achieved with 98% purity.
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