Purpose Endophyte have got much attention as a novel microbial energy source in recent years. Its functions include nitrogen fixation, plant growth promotion and stress resistance of host plants. It is thought that endophyte can produce the same or similarity substances as host plant. Furthermore, some endophytes can also help host plant synthesis more active ingredients.
Method In this study, 23 strains of endophytic fungi were isolated from the tuberous tissue of Paris polyphylla var. yunnanensis, and then cultured with the tissue culture plant of P. polyphylla var.yunnanensis, after culturing for a certain time.
Results Two strains were screened for their increase of saponin content in tissue culture seedlings.
Conclusion Cylindrocarpon sp. and Trichoderma viride can promote the accumulation of active constituent of P. polyphylla var. yunnanensis.