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Feng XIONG, Wei WU, Qianzhuo MAO, et al. Analysis of the Distribution of TVDV in the Alimentary Canal of Myzus persicae by Immunofluorescence[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2018, 33(2): 208-212. DOI: 10.12101/j.issn.1004-390X(n).201705009
Citation: Feng XIONG, Wei WU, Qianzhuo MAO, et al. Analysis of the Distribution of TVDV in the Alimentary Canal of Myzus persicae by Immunofluorescence[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2018, 33(2): 208-212. DOI: 10.12101/j.issn.1004-390X(n).201705009
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Analysis of the Distribution of TVDV in the Alimentary Canal of Myzus persicae by Immunofluorescence

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    • Abstract
  • Purpose The objective of this study was to generate the specific antiserum of TVDV CP and to study the distribution of TVDV in the alimentary canal of Myzus persicae.
    Method The prokaryotic expression vector of pDEST17-TVDV-CP was constructed and transformed into Escherichia coli Rocceta to express the coat protein (CP) of TVDV. The specific antiserum of TVDV CP was obtained through immunizing mice with the purified CP proteins. Then, the TVDV CP antiserum was used to immunolabel TVDV in the transmission vector of M. persicae.
    Result The TVDV CP could be induced at 28 ℃ by 1, 0.5, 0.2 or 0.1 mmol/L IPTG in Rocceta; an about 24 ku protein could be detected using the specific antiserum through analyzing the total protein of TVDV-infected aphids by Western-blot; TVDV were detected in the midgut epithelial cells of M. persicae by immunofluorescence.
    Conclusion The specific antiserum of TVDV CP was generated; TVDV distributed in the midgut epithelial cells of M. persicae, and were transmitted by M. persicae in a circulative manner.
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