Identification and Salicylic Acid Sensitivity Analysis of Arabidopsis Mutant npr1-2

Purpose NPR1 (nonexpresser of pathogenesis-related gene 1) is a key regulator of SAR (systemic acquired resistance) in Arabidopsis thaliana. SAR is an effective innate immune response that provides protection against a broad range of pathogens. Numerous studies have revealed that NPR1 as a salicylic acid (SA) perception chains together extracellular SA signaling and intracellular resistant reaction. Mutant npr1-2 is one of the materials for studying SA signal transduction pathway in Arabidopsis. The objective of this research is to screen out homozygous npr1-2 mutants, detection of NPR1 gene expressional level in homozygous mutants and identification of the sensitivity of mutant seedlings to SA.

Method PCR amplification, DNA sequencing, quantitative RT-PCR detection and SA sensitivity analysis approaches were used in this study.

Result The homozygous npr1-2 mutant was obtained. And the dramatic reduction in expression of NPR1 in the homozygous npr1-2 mutant, to about 30% of wild type plants has been found by qRT-PCR detection. Compared with the wild type, the sensitivity of homozygous npr1-2 seedling roots to high concentration SA was decreased.

Conclusion The homozygous mutant of npr1-2 can provide the material for further research.