Physiological Responses of Tobacco to Salicylic Acid and Sodium Nitroprusside under Waterlogging Stress

Purpose The physiological response of tobacco to salicylic acid (SA) and sodium nitroprusside (SNP) under waterlogging stress was investigated, and the physiological mechanism of SA and SNP to improve the waterlogging tolerance of tobacco was revealed.

Method Through artificial simulation of waterlogging stress, the effect of SA and SNP only and combined treatments on the tobacco root shoot ratio, root activity, chlorophyll content, osmolytes and lipid peroxidation system were studied.

Result Under waterlogging stress, SA and SNP could improve the root shoot ratio and root vigor and slow down the decrease of chlorophyll and carotenoid contents; it could increase the cumulation of soluble sugar, soluble protein and free proline, and enhance the osmotic adjustment ability. SA and SNP could improve SOD and POD activities under short-term waterlogging stress, delay the decrease of the enzyme activities under long-term stress, reduce the content of hydrogen peroxide and oxygen free radical production rate, and further decrease MDA content and relative conductivity, enhance tobacco to waterlogging stress adaptability.

Conclusion The production is available on the SA and SNP combined treatment to alleviate the waterlogging stress on tobacco harm, SA and SNP combined treatment has the best effect.