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Zhengzheng LU, Fang LI. Comparison of the DNA Extraction Effects of Tree Kits on the Tobacco after Baking[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2018, 33(1): 172-175. DOI: 10.12101/j.issn.1004-390X(n).201701025
Citation: Zhengzheng LU, Fang LI. Comparison of the DNA Extraction Effects of Tree Kits on the Tobacco after Baking[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2018, 33(1): 172-175. DOI: 10.12101/j.issn.1004-390X(n).201701025
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Comparison of the DNA Extraction Effects of Tree Kits on the Tobacco after Baking

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    • Abstract
  • Purpose In order to select a kit of simple, fast and efficient to extract the tobacco after baking DNA, we had selected 3 different kits to extract the tobacco after baking DNA.
    Method By comparing the purity and concentration of DNA, quantitative real-time PCR of Ct and gel electrophoresis, we had evaluated the effect.
    Results Using the AxyPrep DNA kit to extract DNA, the purity and concentration of DNA were good, short time to use, the quality was good, apply to extract a little DNA; Using the Magnetic bead DNA kit to extract DNA, the purity and concentration of DNA were slightly higher than the former, but long time to use, suitable to extract lot of DNA; Using the GenePure DNA kit to extract DNA, the purity of DNA were high, but the concentration of DNA were low.
    Conclusion We recommend the use of the AxyPrep DNA kit to extract the tobacco after baking DNA.
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