Cloning and Bioinformatics Analysis of AO Blood Group A Gene in Banna Mini-pig Inbred Line

Purpose To amplify the encoding full-length sequence of AO blood group A gene from Banna mini-pig inbred line and analysis the sequence by bioinformatics software.

Method The tissues RNA from Banna mini-pig inbred line was extracted, and reversely transcribed into cDNA. The gene coding region sequence of AO blood group A gene was amplified through RT-PCR, and the purified product of PCR was ligated with a pMD18-T vector and transferred into the bacterium DH5α for replication. The positive clones were screened and sequenced. The sequence characters were analyzed by using bioinformatics method and phylogeny evolution tree was constructed with other twelve species.

Results The coding region of AO blood group A gene was 1 095 bp, and coded 364 amino acids. The amino acid homology analysis showed that the homology rate of amino acid sequence was 99.5% with that of other species in GenBank.

Conclusions Molecular phylogeny evolution indicated that it had a close relation with yak, Tibetan antelope, buffalo, minke whale, sheep, alpaca, wild camel, goat except chimpanzee, human and mouse, and the most closely related with goat.