Full-length Transcriptome Sequencing of Ericerus pela at the Early Stage of Wax Secretion and Protein Expression Analysis of FAR in Males and Females

PurposeTo understand the gene expression during the early stage of wax secretion in the second-instar males Ericerus pela, and to identify the fatty acyl-CoA reductase (far) gene involved in wax synthesis and the protein expression of FAR in females and males.

MethodThree generations of full-length transcriptome sequencing were used to analyze the genes expression of the second instar males of E. pela during the early stage of wax secretion, and the far genes related to wax secretion of E. pela were identified; monoclonal antibodies were prepared from a concerned FAR. The expression of the FAR in females and males was analyzed by using Western-blot.

ResultThe third generations sequencing of full-length transcriptome was successfully performed on the second-instar males during the early stage of wax secretion of E. pela, a total of 81 far genes were obtained, and 6 far genes with expression profile consistent with the wax secretion of the insect were identified. One of the far genes was selected and named far3. The monoclonal antibody of FAR3 was successfully prepared. And the expression of FAR3 in males and females was analyzed by using the monoclonal antibody, it was confirmed that FAR3 was highly expressed in males during the second larval stage and slightly expressed in females.

ConclusionIn this study, the far genes related to wax secretion were obtained by three generations of full-length transcriptome sequencing of the second-instar males of E. pela during the early stage of wax secretion. The high expression of FAR3 in the second instar males during the wax secretion stage was confirmed by using the monoclonal antibodies of FAR3, which laid a foundation for the functional study of other far genes of E. pela and the exploration of the molecular mechanism of wax secretion of E. pela.