Antibacterial Research on the Bamboo Leaf Extracts from Bambusa cerosissima McClure

PurposeThe antibacterial activity and stability of bamboo leaf extracts from Bambusa cerosissima McClure were studied to provide references for their application.

MethodUsing B. cerosissima as the material and distilled water, ethanol and petroleum ether, chloroform, ethyl acetate and N-butanol as the solvents, the antibacterial effects of bamboo leaf extracts on Staphylococcus aureus, Escherichia coli, Saccharomyces cerevisiae and Aspergillus flavus were studied by the method of bacteriostasis circle. The minimum bacteriostasis mass concentration was determined. The effects of temperature, pH, ultraviolet radiation and chemicals on the antibacterial activity of the extracts were also studied.

ResultsFive bamboo leaf extract systems had different inhibitory effects on four kinds of bacteria, among which the N-butanol extract had the best inhibitory effect on S. aureus with the minimum inhibitory mass concentration of 1.67×10⁻¹ mg/mL. The petroleum ether extract and water soluble extract had the best inhibitory effect on E. coli with the minimum inhibitory mass concentrations of 1.38×10⁻³ mg/mL and 3.17×10^-3 mg/mL, respectively. The butanol extract and water soluble extract had the best bacteriostatic effect on S. cerevisiae with the minimum bacteriostatic mass concentrations of 1.67×10⁻² mg/mL and 3.17×10⁻² mg/mL, respectively, and only the trichloromethane extract had bacteriostatic effect on A. flavus. In addition to Tween 20, the biological activity of bamboo leaf extract is stable for a variety of external conditions.

ConclusionThe extract of bamboo leaves has better bacteriostatic activity, and the biological activity of bamboo leaf extract is relatively stable, so it has a good application prospect.