Abstract: General Chemistry retake course in Yunnan Agricultural University is taken as an example in this paper,which combined with many-year teaching practice of the author himself. From the point of view of the present situation of General Chemistry retake course, the reason why a high ratio and the poor effect of the General Chemistry retake course in agricultural universities is analyzed and discussed. By using the method of literature research and the field investigation, some countermeasures and suggestions, such as the teaching model based on networking question guiding of General Chemistry retake course is put forward, in order to enhance the teaching quality of non-chemistry majors' General Chemistry retake course in agricultural colleges and universities.