云南农业大学学报(自然科学)
    Home     About Journal     Editorial Board     Subscriptions     Download     Message     Contacts Us     Chinese
Journal of Yunnan Agricultural University 2017, Vol. 32 Issue (4) :678-684    DOI: 10.16211/j.issn.1004-390X(n).2017.04.016
Current Issue | Next Issue | Archive | Adv Search << | >>
Studies on Polyploidy Induction of Wild Lilium nepalense D.Don
LI Dan1,2, LUO Yiran1, HAN Guowei1, ZHANG Xue1, WU Yingying1, HE Chengzhong1,3
1. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, China;
2. Yunnan Academy of Biodiversity, Southwest Forestry University, Kunming 650224, China;
3. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China

Abstract
Reference
Related Articles
Download: PDF (1314KB)   HTML 1KB   Export: BibTeX or EndNote (RIS)      Supporting Info
Abstract 

[Purpose] To induce the polyploid and it was expected to obtain new polyploidy germplasm of wild Lilium nepalense.[Methods] The adventitious buds of wild L.nepalense were respectively treated with colchicines by soaking and mixing culture method, and the inducing effects of colchicine at different treatments, mass concentrations, and duration time were investigated. In addition, the variant plants were identified by morphological, stomatal and cytological observations.[Results] The soaking method was applied for polyploid induction, the optimum concentration of colchicine was 0.3%, and the optimum time duration was 12h, with the mutagenic rate as 10.0%. When the mixing culture method was applied for chromosome doubling, the optimum concentration of colchicine was 0.7%, and the mutagenic rate was 46.67%. Through the identification, the tetraploid plants of wild L.nepalense were screened out.[Conclusion] Both of soaking and mixing culture method with colchicine could be used for polyploidy induction of wild L. nepalense, but the effect of mixing culture method with colchicine was better.

Service
Email this article
Add to my bookshelf
Add to citation manager
Email Alert
RSS
Articles by authors
LI Dan
LUO Yiran
HAN Guowei
ZHANG Xue
WU Yingying
HE Chengzhong
KeywordsLilium nepalense D.Don   tissue culture   colchicines   polyploidy induction     
Received 2017-02-07;
About author:
Cite this article:   
LI Dan, LUO Yiran, HAN Guowei, ZHANG Xue, WU Yingying, HE Chengzhong.Studies on Polyploidy Induction of Wild Lilium nepalense D.Don[J]  Journal of Yunnan Agricultural University, 2017,V32(4): 678-684
Copyright 2010 by Journal of Yunnan Agricultural University