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Journal of Yunnan Agricultural University 2017, Vol. 32 Issue (4) :577-581    DOI: 10.16211/j.issn.1004-390X(n).2017.04.002
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Effects of Nitrogen and Wx Gene Deletion on Starch Biosynthesis in Wheat
LI Kunlun1, KONG Zhiyou2, SHA Yun3, TANG Yongsheng4, CHENG Shunhe5, QIN Peng1
1. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China;
2. College of Resources and Environment, Baoshan University, Baoshan 678000, China;
3. Lincang Agricultural Science Institution, Lincang 677000, China;
4. Qujing Agricultural Science Academy, Qujing 655000, China;
5. Lixiahe Region Agricultural Research Institute of Jiangsu Province, Yangzhou 225007, China

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Abstract 

[Purpose] The effects of nitrogen fertilizer level and grain filling stage on the grain starch content of near isogenic Wx wheat lines were determined by different nitrogen application levels.[Method] Eight Wx near-isogenic wheat lines in Ningmai 14 were researched at no basal fertilizer, and 5 nitrogen levels including 0, 150, 300, 450 and 600 kg/hm2 were fertilized at three-leaf period. The amylose, amylopectin and total starch contents of wheat grain were determined at 7, 14, 21, 28 and 35 days after anthesis.[Results] The grain amylose, amylopectin and total starch contents were significantly affected by the Wx genotypes and grain filling period, while the nitrogen levels did not cause significant changes in content and composition of starch. Starch synthesis was speed up 10 d after anthesis till the end of filling period.[Conclusion] The amylose synthesis ability of three Wx genes was Wx-B1 > Wx-D1 > Wx-A1, and the comprehensive effect of Wx-A1 and Wx-D1 was the equivalent of Wx-B1.

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LI Kunlun
KONG Zhiyou
SHA Yun
TANG Yongsheng
CHENG Shunhe
QIN Peng
KeywordsWx near-isogenic wheat lines   nitrogen level   starch   biosynthesis     
Received 2017-02-21;
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LI Kunlun, KONG Zhiyou, SHA Yun, TANG Yongsheng, CHENG Shunhe, QIN Peng.Effects of Nitrogen and Wx Gene Deletion on Starch Biosynthesis in Wheat[J]  Journal of Yunnan Agricultural University, 2017,V32(4): 577-581
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