云南农业大学学报(自然科学)
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Journal of Yunnan Agricultural University 2017, Vol. 32 Issue (4) :571-576    DOI: 10.16211/j.issn.1004-390X(n).2017.04.001
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Cloning and Expression Analyses of Glycosyltransferases Gene OrGT1(t) in Oryza rufupogon
LUO Bi1,2, FENG Jieshen1,2, BAI Heling1,2, LIU Chao1,2, ZHAO Jinlong1,3, TAN Yaling1,2,4, XU Jin1,2,4
1. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China;
2. Rice Research Institute of Yunnan Agricultural University, Kunming 650201, China;
3. Dali Seed Administration Station, Dali 671000, China;
4. Yunnan Engineering Research Center for Japonica Hybrid Rice, Kunming 650201, China

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Abstract [Purpose] In this study, the preliminary study on the glycosyltransferase gene OrGT1(t) in wild rice was carried out, which laid a solid foundation for the further exploration of the synthesis and function of xyloglucan in monocotyledonous plants.[Method] A glycosyltransferases gene OrGT1(t) was cloned from Oryza rufupogon using homologous cloning methods.[Results] Sequence analysis revealed that the gene has a conservative Glyco-transf-34 domain,belonging to the Glyco-transf-34 gene falimy. Phylogenetic analysis showed that OrGT1 may be a xyloglucan xylosetransferase geneinvolve in xyloglucan synthesis.RT-qPCR analysis indicated that the OrGT1 expressed in all checked organs, including the root,stem,leaf and spike in O.rufupogon.But the transcriptional quantity was different among the organs, and there were the highest expression quantity in the stem and the lowest in the root.[Conclusion] The OrGT1 gene is a high degree of functional conservation in the evolutionary process, suggesting that the OrGT1(t) gene may be a xyloglucanase gene involved in xyloglucan synthesis, the results lay a good foundation for further exploring the function of the OrGT1 gene.
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Articles by authors
LUO Bi
FENG Jieshen
BAI Heling
LIU Chao
ZHAO Jinlong
TAN Yaling
XU Jin
KeywordsOryza rufupogon   glycosyltransferase gene   gene clone   real-time PCR     
Received 2017-03-06;
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LUO Bi, FENG Jieshen, BAI Heling, LIU Chao, ZHAO Jinlong, TAN Yaling, XU Jin.Cloning and Expression Analyses of Glycosyltransferases Gene OrGT1(t) in Oryza rufupogon[J]  Journal of Yunnan Agricultural University, 2017,V32(4): 571-576
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