云南农业大学学报(自然科学)
                首 页    期刊介绍        编 委 会        期刊订阅        下载中心        留言板        联系我们        English
云南农业大学学报(自然科学) 2010, Vol. 25 Issue (4) :517-520,534    DOI:
动物科学 最新目录 | 下期目录 | 过刊浏览 | 高级检索 << | >>
金黄色葡萄球菌耐盐生长的形态观察及机理的初步研究
陈俊1, 张惠萍2, 朱希贵3, 黎琼艳1, 陈培富1
1. 云南农业大学动物科学技术学院 云南 昆明 650201;
2. 云南省曲靖市沾益县西平镇畜牧兽医站 云南 曲靖 655331;
3. 云南省玉溪市红塔区春和镇畜牧兽医站 云南 玉溪 653102
Morphological Observation and Pilot Study on Growth Traits of Staphylococcus aureus under High-salt Condition
CHEN Jun1, ZHANG Hui-ping2, ZHU Xi-gui3, LI Qiong-yan1, CHEN Pei-fu1
1. College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;
2. Xiping Husbandry and Veterinary Station,Zhanyi County,Qujing 655331,China;
3. Chunhe Husbandry and Veterinary Station,Hongta District,Yuxi 653102,China

摘要
参考文献
相关文章
Download: PDF (1252KB)   HTML 1KB   Export: BibTeX or EndNote (RIS)      Supporting Info
摘要 为阐明金黄色葡萄球菌特有的耐盐生长特性的机理,试验将该菌分别培养于含低盐(1.0%NaCl)和高盐(10.0%NaCl)的LB液体培养基及卵黄琼脂培养基,观察发现高盐液体条件下该菌呈絮状生长,细菌个体形态明显增大,在高盐固体条件下菌落形态较小,菌落形成单位(CFU)数量也明显减少;对菌体裂解产物进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析,发现金黄色葡萄球菌在高盐与低盐培养条件下的蛋白表达谱存在差异,前者比后者被诱导多表达出至少一个蛋白质条带(MW 22 ku);将相同数量的该菌接种于含0.5 IU/mL青霉素作为细胞壁合成抑制剂及不同氯化钠浓度(1.0%,2.5%,4.0%,5.5%,7.0%,8.5%和10.0%)的卵黄琼脂培养基上培养,发现随着NaCl浓度的升高,CFU数量逐渐减少。结果表明,金黄色葡萄球菌的耐盐生长特性与其细胞壁合成和盐应激蛋白表达有关,并伴随有菌落、菌体形态的变化。
Service
把本文推荐给朋友
加入我的书架
加入引用管理器
Email Alert
RSS
作者相关文章
陈俊
张惠萍
朱希贵
黎琼艳
陈培富*
关键词金黄色葡萄球菌   耐盐生长特性   青霉素   盐应激蛋白     
Abstract: In order to understand mechanisms for peculiar salt-tolerant growth property of Staphylococcus aureus,the bacteria were separately cultured in Luria-Bertani(LB) liquid medium as well as egg yolk agar medium containing low-salt(1.0% NaCl) or high-salt(10.0% NaCl),and it was found that the former grew in a flocculent manner and had a bigger body size in the liquid medium,and showed smaller colony size and produced less colony forming units(CFUs) in the solid medium;the cell lysates from the bacterial cultures were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),resulting in discrepancy of electrophoretic patterns of proteins,namely,at least one more protein band(MW 22 ku) inductively expressed in the high-salt bacterial culture;the bacteria were also cultured on egg yolk agar medium with a final concentration of 0.5 IU/mL penicillin as an inhibitor for cell wall synthesis and different NaCl concentrations(1.0%,2.5%,4.0%,5.5%,7.0%,8.5% and 10.0%),and it turned out that the counting of CFUs decreased with the increase of NaCl concentrations.The findings above demonstrated that intact cell wall and the salt-inducible protein contributed to the salt-tolerance of S.aureus,which in turn made its colonial and individual shapes change.
KeywordsStaphylococcus aureus   salt-tolerance   penicillin   salt-induced protein     
Received 2009-10-10;
Corresponding Authors: 陈培富(1973-),男,博士,副教授,主要从事兽医微生物学与免疫学教学、研究工作.E-mail:cltwins2003@sina.com     Email: cltwins2003@sina.com
About author: 陈俊(1986-),女,江西南康人,主要从事预防兽医学研究.E-mail:jane_now@163.com
引用本文:   
陈俊, 张惠萍, 朱希贵, 黎琼艳, 陈培富.金黄色葡萄球菌耐盐生长的形态观察及机理的初步研究[J]  云南农业大学学报(自然科学), 2010,V25(4): 517-520,534
CHEN Jun, ZHANG Hui-ping, ZHU Xi-gui, LI Qiong-yan, CHEN Pei-fu.Morphological Observation and Pilot Study on Growth Traits of Staphylococcus aureus under High-salt Condition[J]  Journal of Yunnan Agricultural University, 2010,V25(4): 517-520,534
Copyright 2010 by 云南农业大学学报(自然科学)