云南农业大学学报(自然科学)
                首 页    期刊介绍        编 委 会        期刊订阅        下载中心        留言板        联系我们        English
云南农业大学学报(自然科学) 2017, Vol. 32 Issue (4) :600-605    DOI: 10.16211/j.issn.1004-390X(n).2017.04.006
植物保护 最新目录 | 下期目录 | 过刊浏览 | 高级检索 << | >>
小桐子叶斑病菌塔宾曲霉的生物学特性研究
高源1,2,3, 周春香2,4, 杨丽芬2, 杨建2, 杨静1,3, 刘林1,3, 陈吉岳5, 李成云1,3, 郭建伟2,3
1. 云南农业大学, 农业生物多样性应用技术国家工程研究中心, 农业生物多样性与病害控制教育部重点实验室, 云南 昆明 650201;
2. 红河学院 云南省高校农作物优质高效栽培与安全控制重点实验室, 云南 蒙自 661100;
3. 云南生物资源保护与利用国家重点实验室, 云南 昆明 650091;
4. 勐满镇中学, 云南 西双版纳 666206;
5. 中国科学院 西双版纳热带植物园, 云南 勐仑 666303
Study on Biological Characteristics of Aspergillus tubingensis Causing Leaf Spot Disease of Jatropha curcas
GAO Yuan1,2,3, ZHOU Chunxiang2,4, YANG Lifen2, YANG Jian2, YANG Jing1,3, LIU Lin1,3, CHEN Jiyue5, LI Chengyun1,3, GUO Jianwei2,3
1. Key Laboratory for Agri-biodiversity and Pest Management of Education Ministry of China, Key Laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650201, China;
2. Key Laboratory of Higher Quality and Efficient Cultivation and Security Control of Crops for Yunnan Province, Honghe University, Mengzi 661100, China;
3. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Kunming 650091, China;
4. Mengman Middle School, Xishuangbanna 666206, China;
5. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun 666303, China

摘要
参考文献
相关文章
Download: PDF (918KB)   HTML 1KB   Export: BibTeX or EndNote (RIS)      Supporting Info
摘要 

[目的]探讨碳源、氮源、温度、pH值、光照等对小桐子叶斑病塔宾曲霉(Aspergillus tubuingensis Moss.)的影响,为其防治提供理论依据。[方法]采用十字交叉法从碳源、氮源、温度、pH值、光照等方面对塔宾曲霉菌丝生长、产孢量的影响进行研究。[结果]塔宾曲霉在尿素、甘氨酸为唯一氮源之外的其他17种培养基上均能良好的生长,菌丝生长最佳碳氮源为甘露醇、牛肉膏;菌丝在23~37℃均能生长,最适温度为35℃,致死温度为65℃;培养基中pH值4~8时均能生长,最适pH值为8;此外,光照能促进菌丝生长。塔宾曲霉在尿素为唯一氮源之外的其他18种培养基上均能产孢,产孢最适碳氮源分别为可溶性淀粉、牛肉膏;在23~37℃均能产孢,最适温度35℃;培养基中pH值4~8时均能产孢,最适pH值为6;光照对孢子生长有促进作用。[结论]塔宾曲霉对环境的适应能力较强,对酸碱度敏感,可能与寄主小桐子的生长环境及叶片的生理生化性质有关。

Service
把本文推荐给朋友
加入我的书架
加入引用管理器
Email Alert
RSS
作者相关文章
高源
周春香
杨丽芬
杨建
杨静
刘林
陈吉岳
李成云
郭建伟
关键词塔宾曲霉   生物学特性   小桐子叶斑病     
Abstract

[Purpose] The objective of this study was to provide theoretical foundation on prevention and control of Aspergillus tubuingensis Moss. causing leaf spot disease on Jatropha curcas L.[Method] The effect of carbon sources, nitrogen sources, temperature, pH value, light on the mycelia growth and conidial production was studied with crossing method.[Results] A. tubuingensis could grow on 17 kinds of solid media except for urea and glycine and the most suitable carbon and nitrogen source for mycelia growth were mannitol and beef extract, respectively. The fungus grew well between 23-37℃, the most suitable temperature was 35℃, the lethal temperature was 65℃; the fungus could grow between 4-8 of pH value, the most suitable pH value was 8, and light promoted the growth of mycelia. The fungus could produce conidia on 18 kinds of solid media except for urea, the most suitable carbon and nitrogen source for reproduction spore were starch and beef extract respectively; it could produce conidia between 23-37℃, the most suitable temperature was 35℃; it also could produce conidia at the regime of 4-8 of pH value, the most suitable pH value was 6, light promoted the reproduction of spore.[Conclusions] The results demonstrated that A. tubuingensis had a strong ability to adapt the environment, but it was sensitive to pH, which is related to environment of J. curcas and biological and biochemical characteristics of Jatropha leaves.

KeywordsAspergillus tubingensis   biological characteristics   leaf spot disease of Jatropha curcas     
Received 2017-01-24;
Fund:

红河学院中青年学术骨干培养计划(2015GG0206);红河学院硕博科研启动专项项目(XJ15B16);红河学院应用型研究项目(XJY15Y02);中山大学广东省热带亚热带植物资源重点实验室开放课题(PlantKF201503);红河学院植物保护一级学科硕士点建设项目;国家重点研发计划项目(2016YFC0501500)。

About author: 高源(1985-),男,云南蒙自人,在读博士研究生,主要从事植物病原真菌鉴定及其防治研究。E-mail:454883064@qq.com;周春香(1988-),女,云南曲靖人,学士,主要从事植物病原菌鉴定研究。E-mail:364929774@qq.com
引用本文:   
高源, 周春香, 杨丽芬, 杨建, 杨静, 刘林, 陈吉岳, 李成云, 郭建伟.小桐子叶斑病菌塔宾曲霉的生物学特性研究[J]  云南农业大学学报(自然科学), 2017,V32(4): 600-605
GAO Yuan, ZHOU Chunxiang, YANG Lifen, YANG Jian, YANG Jing, LIU Lin, CHEN Jiyue, LI Chengyun, GUO Jianwei.Study on Biological Characteristics of Aspergillus tubingensis Causing Leaf Spot Disease of Jatropha curcas[J]  Journal of Yunnan Agricultural University, 2017,V32(4): 600-605
Copyright 2010 by 云南农业大学学报(自然科学)