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云南农业大学学报(自然科学) 2017, Vol. 32 Issue (4) :740-746    DOI: 10.16211/j.issn.1004-390X(n).2017.04.026
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PCR-DGGE分析玉溪地区水果泡菜中细菌多样性
王蓓1,2, 李淑英1, 张翠萍1, 卢国理1, 周元清1
1. 玉溪师范学院, 污染控制与生态修复研究中心, 云南 玉溪 653100;
2. 云南大学, 生态学与地植物学研究所, 云南 昆明 650091
PCR-DGGE Analysis for Bacterial Diversity of Fruit Pickles in Yuxi Region
WANG Bei1,2, LI Shuying1, ZHANG Cuiping1, LU Guoli1, ZHOU Yuanqing1
1. Research Center for Pollution Control and Ecological Restoration, Yuxi Normal University, Yuxi 653100, China;
2. Institute of Ecology and Geobotany, Yunnan University, Kunming 650091, China

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摘要 

[目的]结合自然发酵生产泡菜的原理,分析云南传统水果泡菜的细菌群落组成和结构多样性,探索泡菜发酵过程中的微生物机制。[方法]采用PCR-DGGE和构建16S rRNA基因文库的方法,对从玉溪市场购买的萝卜、黄瓜、梨3种泡菜样品中细菌群落结构进行研究。[结果]玉溪地区自然发酵泡菜中的细菌种类比较丰富,由于不同水果泡菜的营养基质和附着在蔬菜表面及内在的细菌群落结构不同,导致细菌种类在不同泡水果中所占的相对丰度有较大差异。乳酸菌属(Lactobacillus sp.)在泡水果中并非优势种群,但丘状乳杆菌(Lactobacillus collinoides)在发酵中发挥着重要的作用。此外,在散称泡菜液中检测出部分致病菌,主要为肠球菌属(Enterococcus sp.)、葡萄球菌属(Staphylococcus sp.)和不动杆菌属(Acinetobacter sp.),推测市场上散称泡菜容易滋生致病菌,可能存在一定安全风险。[结论]PCR-DGGE技术能够高效、精确地检测到水果泡菜的细菌多样性。泡菜微生物的组成是指示泡菜安全性的一个重要指标。

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王蓓
李淑英
张翠萍
卢国理
周元清
关键词水果泡菜   细菌多样性   16S rRNA基因   变性梯度凝胶电泳(DGGE)     
Abstract

[Purpose] Based on the principle of natural fermentation, this study is to analyze the bacterial composition and diversity of traditional fruit pickle in Yunnan.[Method] Three kinds of different materials of pickles(radish, cucumber and pear)were studied based on the analysis of PCR-DGGE and construction of 16S rRNA gene library.[Results] The bacterial species of Yuxi natural fermentation pickles were rich, Lactobacillus collinoides played an important role in the fruit pickle. And there were large diversity relative abundance of bacterial phylogenetic groups identified in radish, cucumber and pear pickles, due to nutrient medium and bacterial community structure attached to the surface and inner of the three fruit pickles were different. In addition, we detected part of pathogens comprising Enterococcus sp., Staphylococcus sp. and Acinetobacter sp. in the fermentation broth, which can reasonably inferred that the part of pickles purchased scattered on is easy to breed pathogenic bacteria and may exist the potential security problems.[Conclusion] PCR-DGGE is an effective and accurate technique in analyzing microbial diversity.The composition of microorganisms is an important indication for pickles' safety.

Keywordsfruits pickles   bacterial diversity   16S rRNA gene   denaturing gradient gel electrophoresis (DGGE)     
Received 2016-10-26;
Fund:

国家自然科学基金项目(31460144);云南省教育厅重点项目(2014Z148)。

Corresponding Authors: 周元清(1974-),女,云南玉溪人,博士,教授,硕士生导师,主要从事污染控制与生态修复研究。E-mail:yqzhou@yxnu.net     Email: yqzhou@yxnu.net
About author: 王蓓(1992-),女,陕西渭南人,在读硕士研究生,主要从事污染控制与生态修复研究。E-mail:2536882707@qq.com
引用本文:   
王蓓, 李淑英, 张翠萍, 卢国理, 周元清.PCR-DGGE分析玉溪地区水果泡菜中细菌多样性[J]  云南农业大学学报(自然科学), 2017,V32(4): 740-746
WANG Bei, LI Shuying, ZHANG Cuiping, LU Guoli, ZHOU Yuanqing.PCR-DGGE Analysis for Bacterial Diversity of Fruit Pickles in Yuxi Region[J]  Journal of Yunnan Agricultural University, 2017,V32(4): 740-746
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