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云南农业大学学报(自然科学) 2017, Vol. 32 Issue (4) :678-684    DOI: 10.16211/j.issn.1004-390X(n).2017.04.016
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野生紫斑百合多倍体诱导研究
李旦1,2, 罗一然1, 韩国伟1, 张雪1, 吴英英1, 何承忠1,3
1. 西南林业大学, 云南省高校林木遗传改良与繁育重点实验室, 云南 昆明 650224;
2. 西南林业大学, 云南生物多样性研究院, 云南 昆明 650224;
3. 西南林业大学, 西南山地森林资源保护与利用教育部重点实验室, 云南 昆明 650224
Studies on Polyploidy Induction of Wild Lilium nepalense D.Don
LI Dan1,2, LUO Yiran1, HAN Guowei1, ZHANG Xue1, WU Yingying1, HE Chengzhong1,3
1. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, China;
2. Yunnan Academy of Biodiversity, Southwest Forestry University, Kunming 650224, China;
3. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China

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摘要 

[目的]对野生紫斑百合丛生芽进行多倍体诱导,以期获得多倍体新种质。[方法]以野外采集的野生紫斑百合为材料,以秋水仙素为诱变剂,分别采用浸泡法和混培法处理野生紫斑百合试管苗不定芽,比较分析不同处理方式、不同质量浓度及不同处理时间的秋水仙素对野生紫斑百合的诱变效果,并通过形态、气孔和细胞学观测鉴定变异植株。[结果]用浸泡法进行多倍体诱导,秋水仙素的最佳浓度为0.3%,最佳处理时间为12 h,变异率达到10.0%;用秋水仙素混培法进行染色体加倍的最佳浓度为0.7%,诱变率达到46.67%。通过鉴定,初步筛选出了野生紫斑百合的四倍体植株。[结论]秋水仙素的浸泡法和混培法均可诱导获得野生紫斑百合的多倍体,但以混培法诱导效果较好。

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李旦
罗一然
韩国伟
张雪
吴英英
何承忠
关键词紫斑百合   组织培养   秋水仙素   多倍体诱导     
Abstract

[Purpose] To induce the polyploid and it was expected to obtain new polyploidy germplasm of wild Lilium nepalense.[Methods] The adventitious buds of wild L.nepalense were respectively treated with colchicines by soaking and mixing culture method, and the inducing effects of colchicine at different treatments, mass concentrations, and duration time were investigated. In addition, the variant plants were identified by morphological, stomatal and cytological observations.[Results] The soaking method was applied for polyploid induction, the optimum concentration of colchicine was 0.3%, and the optimum time duration was 12h, with the mutagenic rate as 10.0%. When the mixing culture method was applied for chromosome doubling, the optimum concentration of colchicine was 0.7%, and the mutagenic rate was 46.67%. Through the identification, the tetraploid plants of wild L.nepalense were screened out.[Conclusion] Both of soaking and mixing culture method with colchicine could be used for polyploidy induction of wild L. nepalense, but the effect of mixing culture method with colchicine was better.

KeywordsLilium nepalense D.Don   tissue culture   colchicines   polyploidy induction     
Received 2017-02-07;
Fund:

云南省环保专项资金项目(2014BI0011);云南省教育厅重大专项项目(ZD2013014);云南省林学一流学科建设项目(51600625);西南林业大学大学生创新基金项目(C16013,C15066)。

Corresponding Authors: 何承忠(1970-),男,甘肃民勤人,博士,教授,主要从事林木遗传育种与分子生物学研究。E-mail:hcz70@163.com     Email: hcz70@163.com
About author: 李旦(1985-),女,吉林四平人,博士,助理研究员,主要从事植物遗传育种研究。E-mail:lidan_163@163.com
引用本文:   
李旦, 罗一然, 韩国伟, 张雪, 吴英英, 何承忠.野生紫斑百合多倍体诱导研究[J]  云南农业大学学报(自然科学), 2017,V32(4): 678-684
LI Dan, LUO Yiran, HAN Guowei, ZHANG Xue, WU Yingying, HE Chengzhong.Studies on Polyploidy Induction of Wild Lilium nepalense D.Don[J]  Journal of Yunnan Agricultural University, 2017,V32(4): 678-684
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