Studies on Polyploidy Induction of Wild Lilium nepalense D.Don
LI Dan1,2, LUO Yiran1, HAN Guowei1, ZHANG Xue1, WU Yingying1, HE Chengzhong1,3
1. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, China;
2. Yunnan Academy of Biodiversity, Southwest Forestry University, Kunming 650224, China;
3. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China
[Purpose] To induce the polyploid and it was expected to obtain new polyploidy germplasm of wild Lilium nepalense.[Methods] The adventitious buds of wild L.nepalense were respectively treated with colchicines by soaking and mixing culture method, and the inducing effects of colchicine at different treatments, mass concentrations, and duration time were investigated. In addition, the variant plants were identified by morphological, stomatal and cytological observations.[Results] The soaking method was applied for polyploid induction, the optimum concentration of colchicine was 0.3%, and the optimum time duration was 12h, with the mutagenic rate as 10.0%. When the mixing culture method was applied for chromosome doubling, the optimum concentration of colchicine was 0.7%, and the mutagenic rate was 46.67%. Through the identification, the tetraploid plants of wild L.nepalense were screened out.[Conclusion] Both of soaking and mixing culture method with colchicine could be used for polyploidy induction of wild L. nepalense, but the effect of mixing culture method with colchicine was better.