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云南农业大学学报(自然科学) 2017, Vol. 32 Issue (4) :620-626    DOI: 10.16211/j.issn.1004-390X(n).2017.04.009
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大白母猪LIF基因多态性及其对繁殖性状的影响
刘碧霞1, 杨善海1,2, 严达伟1, 李中昌3, 张燕林3, 计春律3, 董新星1, 兰国湘1, 李明丽1, 鲁绍雄1
1. 云南农业大学 动物科学技术学院, 云南 昆明 650201;
2. 云南省德宏州芒市农业局畜牧站, 云南 芒市 678400;
3. 云南惠嘉育种有限公司, 云南 昆明 650300
Polymorphisms of LIF Gene and Their Effects on Reproductive Traits in Large White Sows
LIU Bixia1, YANG Shanhai1,2, YAN Dawei1, LI Zhongchang3, ZHANG Yanlin3, JI Chunlyu3, DONG Xinxing1, LAN Guoxiang1, LI Mingli1, LU Shaoxiong1
1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
2. Animal Husbandry Station, Mangshi Agricultural Buareau, Dehong Prefecture, Yunnan Province, Mangshi 678400, China;
3. Yunnan Huijia Breeding Company Limited, Kunming 650300, China

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摘要 

[目的]为揭示白血病抑制因子(leukemia inhibitory factor,LIF)基因变异位点与母猪繁殖性状的关联。[方法]采用PCR产物直接测序法检测了405头大白母猪LIF基因C4950G和C6988T位点的多态性,结合1716窝母猪繁殖性能记录,分析了单个位点基因型及两位点合并基因型对5个繁殖性状的影响。[结果]大白猪C4950G和C6988T位点分别以等位基因G和T的频率最高,且均处于Hardy-Weinberg平衡状态,杂合度较高。C4950G位点GG基因型母猪的总产仔数、产活仔数、仔猪初生窝重及21日龄成活数显著高于其余两种基因型(P<0.05或P<0.01),C6988T位点CT基因型母猪上述4个性状显著高于TT基因型(P<0.05)。两个位点对母猪繁殖性能的影响呈现了明显的协同作用,合并基因型为GGCT的母猪,可较其他基因型显著提高总产仔数0.90~1.05头(P<0.05或P<0.01)、产活仔数0.54~0.88头(P<0.05或P<0.01)、仔猪21日龄成活数1.02~1.95头(P<0.05或P<0.01),仔猪初生窝重与21日龄窝重也更大(P<0.05或P<0.01)。[结论]进一步证实LIF基因与母猪繁殖性状间存在显著关联,但在不同品种(猪群)中,该基因的具体效应可能会有所不同。

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刘碧霞
杨善海
严达伟
李中昌
张燕林
计春律
董新星
兰国湘
李明丽
鲁绍雄
关键词大白猪   白血病抑制因子   单核苷酸多态性   繁殖性状     
Abstract

[Purpose] The aim of this study was to determine the associations between the mutations in leukemia inhibitory factor (LIF) gene and the reproductive traits of sows.[Method] The polymorphisms of C4950G and C6988T within LIF gene were examined by PCR product directed sequencing in 405 Large White pigs. Combining the data of 1716 litters reproductive performance, the effects of genotypes for single locus and combined genotypes for bi-loci on 5 reproductive traits were analyzed.[Result] The allele with the highest frequency at locus C4950G and C6988T was G and T, respectively, both loci were in Hardy-Weinberg equilibrium and had a higher heterozygosity. At the C4950G locus, sows of GG genotype had higher total number born (TNB), number of piglets born alive (NBA), litter weight at birth (LWB) and number of alive piglets on 21st day (N21) when compared to the other two genotype (P<0.05 or P<0.01); and at the C6988T locus, the above traits of sows with CT genotype were significantly higher than those of TT genotype (P<0.05). The obvious synergistic action was showed on the effects of the two loci on the sow reproduction traits. Compared to other combined genotypes, TNB, NBA and N21 of the sows with genotype GGCT increased 0.90-1.05, 0.54-0.88 and 1.02-1.95 piglets, respectively (P<0.05 or P<0.01), together with larger LWB and litter weight of piglets on 21st day (P<0.05 or P<0.01).[Conclusion] The present results further confirmed the significant associations of LIF gene with sow reproductive traits, but its specific effects would be variety in different pig breeds or populations.

KeywordsLarge White pig   leukemia inhibitory factor (LIF)   single nucleotide polymorphism (SNP)   reproductive trait     
Received 2017-03-14;
Fund:

NSFC-云南联合基金项目(U1402266);云南省高校科技创新团队支持计划(云教科[2014]22号);云南省现代农业生猪产业技术体系建设项目(2017KJTX0013)。

Corresponding Authors: 李明丽(1969-),女,云南富民人,硕士,副教授,主要从事动物生产研究。E-mail:xiaolucao@126.com;鲁绍雄(1972-),男,云南镇雄人,博士,教授,博士生导师,主要从事动物遗传育种研究。E-mail:shxlu@263.net     Email: xiaolucao@126.com;shxlu@263.net
About author: 刘碧霞(1987-),女,山西朔州人,硕士研究生,主要从事动物遗传育种研究。E-mail:1326392152@qq.com;杨善海(1979-),男,云南芒市人,本科,兽医师,主要从事畜牧兽医技术推广工作。E-mail:lxxmyangshanhai@163.com
引用本文:   
刘碧霞, 杨善海, 严达伟, 李中昌, 张燕林, 计春律, 董新星, 兰国湘, 李明丽, 鲁绍雄.大白母猪LIF基因多态性及其对繁殖性状的影响[J]  云南农业大学学报(自然科学), 2017,V32(4): 620-626
LIU Bixia, YANG Shanhai, YAN Dawei, LI Zhongchang, ZHANG Yanlin, JI Chunlyu, DONG Xinxing, LAN Guoxiang, LI Mingli, LU Shaoxiong.Polymorphisms of LIF Gene and Their Effects on Reproductive Traits in Large White Sows[J]  Journal of Yunnan Agricultural University, 2017,V32(4): 620-626
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