Cloning,Molecular Characterization and Tissue Expression Profile of Binglangjiang Buffalo Gene Dmrt7
WU Shan1, JI Li2, ZHANG Yongyun3, DU Ping4, KANG Dingfu4, HA Fu1, MIAO Yongwang1
1. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
2. Department of Animal Husbandry & Veterinary Medicine, Yunnan Agricultural College of Vocational Education, Kunming 650212, China;
3. Teaching Demonstration Center of the Basic Experiments of Agricultural Majors, Yunnan Agricultural University, Kunming 650201, China;
4. Tengchong Co., Ltd. of Fine Variety Breeding of Binglangjiang Buffalo, Tengchong 679118, China
[Purposes] As one of the members of Dmrt gene family, Dmrt7 gene affects the spermiogenesis of male animals. So far, few studies have been done related to the genetic basis of buffalo spermiogenesis. In this study, by employed Binglangjiang water buffalo as research object, the complete CDS sequence of water buffalo Dmrt7 gene was cloned, and its sequence characterization, tissue differential expression were assayed and analysed.[Methods] RT-PCR and functional bioinformatics methods were used.[Results] The results showed that the CDS of Binglangjiang buffalo Dmrt7 gene is 1113 bp in length, which encodes a polypeptide composed of 370 amino acids. Buffalo Dmrt7 contains two conserved domains, viz. a DM and a DMRT-like domain. This protein was predicted a nuclear protein without signal peptide and transmembrane domain. Homology analysis showed that the amino acid sequence similarity of Dmrt7 between buffalo and other bovine species was more than 95%. Among 10 different tissues of adult buffalo, Dmrt7 gene was detected to only express in the testis of water buffalo, and its expression level was dominantly high. The polymorphism c.665A>G was found in exon 6 of CDS of buffalo Dmrt7 gene which leads to a p.Q222R change. But this substitution was presumed to have no functional effect on buffalo Dmrt7 protein.[Conclusion] The results of this study will provide fundamental insights into the function of buffalo Dmrt7 gene.
WU Shan, JI Li, ZHANG Yongyun, DU Ping, KANG Dingfu, HA Fu, MIAO Yongwang.Cloning,Molecular Characterization and Tissue Expression Profile of Binglangjiang Buffalo Gene Dmrt7[J] Journal of Yunnan Agricultural University, 2017,V32(4): 612-619