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云南农业大学学报(自然科学) 2017, Vol. 32 Issue (3) :442-449    DOI: 10.16211/j.issn.1004-390X(n).2017.03.008
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版纳微型猪近交系细胞色素P450家族基因CYP1A1克隆及生物信息学分析
王淑燕1,2, 王配1,2, 霍金龙1,2, 查星琴1,2, 潘伟荣1,2, 张霞1,2, 王雪飞2
1. 云南农业大学, 云南省版纳微型猪近交系重点实验室, 云南 昆明 650201;
2. 云南农业大学 动物科学技术学院, 云南 昆明 650201
Cloning and Bioinformatics Analysis of CYP1A1 of Cytochrome P450 Family from Banna Mini-pig Inbred Line
WANG Shuyan1,2, WANG Pei1,2, HUO Jinlong1,2, ZHA Xingqin1,2, PAN Weirong1,2, ZHANG Xia1,2, WANG Xuefei2
1. Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China;
2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China

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摘要 [目的]克隆版纳微型猪近交系(Banna mini-pig inbred line;BMI)细胞色素P450家族基因CYP1A1的编码区序列,并对其进行生物信息学分析。[方法] 利用RT-PCR方法获得CYP1A1基因的全长CDS序列,运用生物信息学对其核酸及蛋白序列进行分析,构建系统进化树。[结果] 获得的BMI CYP1A1 CDS长度为1 551 bp,编码516个氨基酸,蛋白质分子量为58.39 ku,等电点为7.83,位于内质网的概率是94.1%。CYP1A1蛋白质含有1个细胞色素P450半胱氨酸血红素配体位点,1个N-豆蔻酰化位点,1个酰胺化作用位点,2个N-糖基化位点、6个蛋白酶C磷酸化位点和5个蛋白激酶Ⅱ磷酸化位点。11个物种构建的系统进化分析表明,BMI(猪)CYP1A1与牛和山羊的关系最近。[结论] 以上结果将为进一步研究版纳微型猪近交系CYP1A1基因功能奠定基础。
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王淑燕
王配
霍金龙
查星琴
潘伟荣
张霞
王雪飞
关键词细胞色素P450家族   CYP1A1基因   编码区序列   版纳微型猪近交系   生物信息学   药物代谢     
Abstract: [Purpose] Our purpose was to clone sequence and analyze the character of the cytochrome P450 family 1 subfamily A member 1 gene (CYP1A1) from Banna mini-pig inbred line (BMI).[Methods] The complete CDS of CYP1A1 gene was cloned using RT-PCR method from BMI. Nucleotide and protein sequences of CYP1A1 were utilized to carry out bioinformatics analysis and construct the phylogenetic tree.[Results] The CDS length of BMI CYP1A1 is 1 551 bp in length which encodes a protein of 516 amino acids with molecular weight (Mw) 58.39 ku, and isoelectric point (pI) 7.83, and locates in the endoplasmic reticulum with 94.1% reliability. CYP1A1 protein contains a cytochrome P450 cysteine heme-iron ligand signature, an N-myristoylation site, a aidation site, two N-glycosylation sites, six protease C phosphorylation sites and five protein kinase Ⅱ phosphorylation sites. Phylogenetic analysis of 11 species showed that CYP1A1 of BMI (pig) had the closest relationship with these of cattle and goat.[Conclusion] The results will lay a foundation for further study on CYP1A1 gene function in BMI.
Keywordscytochrome P450 family   CYP1A1 gene   CDS   banna mini-pig inbred line (BMI)   bioinformatics   drug metabolism     
Received 2016-12-19;
Fund:国家自然科学基金项目(31660637,31460580,31660650)。
Corresponding Authors: 霍金龙(1975-),男,山西五寨人,博士,副教授,主要从事动物分子遗传学研究。E-mail:jinlonghuo973@163.com     Email: jinlonghuo973@163.com
About author: 王淑燕(1987-),女,山东济南人,硕士,实验师,主要从事动物分子遗传学研究。E-mail:shuyanwang2014@126.com;王配(1987-),女,河南上蔡人,博士,实验师,主要从事动物分子生物学研究。E-mail:peipei99999@126.com
引用本文:   
王淑燕, 王配, 霍金龙, 查星琴, 潘伟荣, 张霞, 王雪飞.版纳微型猪近交系细胞色素P450家族基因CYP1A1克隆及生物信息学分析[J]  云南农业大学学报(自然科学), 2017,V32(3): 442-449
WANG Shuyan, WANG Pei, HUO Jinlong, ZHA Xingqin, PAN Weirong, ZHANG Xia, WANG Xuefei.Cloning and Bioinformatics Analysis of CYP1A1 of Cytochrome P450 Family from Banna Mini-pig Inbred Line[J]  Journal of Yunnan Agricultural University, 2017,V32(3): 442-449
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