云南农业大学学报(自然科学)
                首 页    期刊介绍        编 委 会        期刊订阅        下载中心        留言板        联系我们        English
云南农业大学学报(自然科学) 2017, Vol. 32 Issue (2) :226-232    DOI: 10.16211/j.issn.1004-390X(n).2017.02.006
植物保护 最新目录 | 下期目录 | 过刊浏览 | 高级检索 << | >>
金龟子绿僵菌MAE921与Bt配伍对铜绿丽金龟幼虫侵染致病效应研究
刘思雨1, 户艳霞2, 王新中2, 徐发华2, 杜广祖1, 李秀军3, 陈斌1, 罗云方4
1. 云南农业大学 植物保护学院, 云南 昆明 650201;
2. 云南省烟草公司大理州公司, 云南 大理 651000;
3. 昆明红桑科技有限责任公司, 云南 昆明 650224;
4. 大理州烟草公司云龙县分公司, 云南 云龙 672700
The Infection Effect of Metarhizium anisopliae MAE921 with Bt against 2nd instar Larvae of the Anomala corpulenta Motschulsky
LIU Siyu1, HU Yanxia2, WANG Xinzhong2, XU Fahua2, DU Guangzu1, LI Xiujun3, CHEN Bin1, LUO Yunfang4
1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;
2. Dali Prefecture in Yunnan Province Tobacco Company, Dali 671000, China;
3. Kunming Hongsang Science Co., Ltd., Kunming 650224, China;
4. Dali Prefecture Tobacco Company of Yunlong County Branch Company, Yunlong 672700, China

摘要
参考文献
相关文章
Download: PDF (1508KB)   HTML 1KB   Export: BibTeX or EndNote (RIS)      Supporting Info
摘要 为了明确金龟子绿僵菌与Bt可湿性粉剂配伍后对铜绿丽金龟幼虫的侵染致病的协同增效作用,为该害虫的防治提供新途径,室内生物测定了金龟子绿僵菌MAE921与Bt制剂配伍对铜绿丽金龟2龄幼虫的侵染致病效应。结果表明:绿僵菌MAE921与Bt可湿性粉剂配伍后能提高绿僵菌对铜绿丽金龟2龄幼虫的侵染致病作用,死亡率随接种浓度的升高而增加,其中绿僵菌MAE921分别与0.1、0.05和0.025 g/mL Bt可湿性粉剂配伍接种后第5、7天的LC50对数估计值分别为5.152 7、5.197 8和5.984 6,3.797 7、3.906 2和4.517 4,均明显低于绿僵菌纯菌剂第5、7天的LC50对数估计值6.472 7和4.806 3。当用1.42×105 mL-1绿僵菌分别与0.1、0.05、0.025 g/mL Bt配伍后,对铜绿丽金龟2龄幼虫的LT50分别为5.00、5.05和5.80 d,均小于纯菌剂LT50F<0.05);高剂量1.42×108 mL-1绿僵菌与0.1、0.05和0.025 g/mL Bt配置接种后,LT50分别为2.65、2.96和3.62 d,与纯孢子悬浮液的LT50间无显著差异(F>0.05)。由此表明:绿僵菌MAE921与Bt可湿性粉剂配伍对侵染铜绿丽金龟2龄幼虫具有良好的协同增效作用,其侵染致病效应符合“时间-剂量-死亡率模型(TDM)”。
Service
把本文推荐给朋友
加入我的书架
加入引用管理器
Email Alert
RSS
作者相关文章
刘思雨
户艳霞
王新中
徐发华
杜广祖
李秀军
陈斌
罗云方
关键词金龟子绿僵菌   铜绿丽金龟   苏云金芽孢杆菌Bt   协同增效作用   时间-剂量-死亡率(TDM)模型     
Abstract: To confirm the synergism effect,and get a new method for the control of the larvae of Anomala corpulenta, the entomopathogenic fungi Metarhizium anisopliae combined with Bacillus thuringiensis agent, bioassay test against the 2nd instar larvae of A. corpulenta was conducted in the laboratory using the conidia suspension of M. anisopliae MAE921 combined with Bt at the dosage of 0.1,0.05 and 0.025g/mL. Results showed that the combination of M. anisopliae MAE921 with Bt showed a higher synergism against the 2nd instar larvae of A. corpulenta, the mortality increased with the increase of the inoculation concentration,the logarithm value of LC50 were 5.152 7,5.197 8 and 5.984 6 on the 5th day,3.797 7,3.906 2 and 4.517 4 on the 7th day after the inoculation of MAE921 with Bt at 0.1, 0.05 and 0.025 g/mL, respectively, they were all lower than the logarithm value of LC50 as that 6.472 7 and 4.806 3. The LT50 were 5.00,5.05 and 5.80 days after inoculating the M. anisopliae MAE921 at the concentration of 1.42×105 mL-1 combined with Bt at 0.1,0.05, and 0.025 g/mL, respectively,they were all lower than the LT50 from the M. anisopliae MAE921(F<0.05). The LT50 were 2.65,2.96 and 3.62 days after inoculating the M. anisopliae at higher concentration of 1.42×108 mL-1 with 0.1,0.05 and 0.025 g/mL Bt, but no significant difference was found between them and those LT50 from the combination of 1.42×108 mL-1 M. anisopliae with Bt at 0.1,0.05 and 0.025 g/mL Bt(F<0.05). All the results showed that M. anisopliae combined with B. thuringiensis can get a good synergism against the 2nd instar larvae of A. corpulenta, and the infection effect was in accord with the time-dose-mortality model.
KeywordsMetarhizium anisopliae   Anomala corpulenta   Bacillus thuringiensis   synergistic effect   time-dose-mortality (TDM)modeling     
Received 2015-10-12;
Fund:云南省烟草公司项目(2014YN29)。
Corresponding Authors: 王新中(1978-),男,河南焦作人,博士,农艺师,主要从事烟草栽培研究。E-mail:xzhang@163.com;陈斌(1970-),男,甘肃礼县人,博士,教授,主要从事农业昆虫与害虫综合治理教学与科研。E-mail:chbins@163.com     Email: xzhang@163.com;chbins@163.com
About author: 刘思雨(1991-),男,陕西扶风人,硕士研究生,主要从事害虫综合防治研究。E-mail:779350009@qq.com
引用本文:   
刘思雨, 户艳霞, 王新中, 徐发华, 杜广祖, 李秀军, 陈斌, 罗云方.金龟子绿僵菌MAE921与Bt配伍对铜绿丽金龟幼虫侵染致病效应研究[J]  云南农业大学学报(自然科学), 2017,V32(2): 226-232
LIU Siyu, HU Yanxia, WANG Xinzhong, XU Fahua, DU Guangzu, LI Xiujun, CHEN Bin, LUO Yunfang.The Infection Effect of Metarhizium anisopliae MAE921 with Bt against 2nd instar Larvae of the Anomala corpulenta Motschulsky[J]  Journal of Yunnan Agricultural University, 2017,V32(2): 226-232
Copyright 2010 by 云南农业大学学报(自然科学)