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云南农业大学学报(自然科学) 2017, Vol. 32 Issue (1) :78-82    DOI: 10.16211/j.issn.1004-390X(n).2017.01.011
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月月粉(Rosa chinensis ‘Pallida’)、大马士革蔷薇(R. damascene)、百叶蔷薇(R. centifolia)香气成分分析
晏慧君1, 王娟2, 陈敏1, 王其刚1, 张颢1
1. 云南省农业科学院 花卉研究所, 云南 昆明 650205;
2. 云南师范大学文理学院, 云南 昆明 650224
The Analysis of Scent Compounds from Rosa chinensis ‘Pallida’, R. damascene and R. centifolia by GC/MS
YAN Huijun1, WANG Juan2, CHEN Min1, WANG Qigang1, ZHANG Hao1
1. Flower Research Institute, Yunnan Agriculture Academy Sciences, Kunming 650205, China;
2. The College of Art and Sciences, Yunnan Normal University, Kunming 650224, China

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摘要 

以月季资源月月粉、大马士革蔷薇和百叶蔷薇为材料,采用顶空固相微萃取技术,比较分析了其盛开期花瓣和雄蕊香气成分差异。结果表明:不同材料及同一材料的花瓣和雄蕊香味物质的种类及相对含量差别明显。月月粉的主要香气物质是芳香烃、萜烯类,其中,1,3,5-三甲氧基苯是其特征香味成分;大马士革和百叶蔷薇的主要香味成分组成基本一致,花瓣主要成分是2-苯乙醇,相对含量约占69%,二者微量香气差别成分是榄香醇、甲基丁香酚、香叶酯;丁香酚在3种材料的雄蕊中均存在,且是大马士革和百叶蔷薇的主要成分,而在花瓣中不含有。此研究结果为从这些月季资源中克隆相应的关键酶基因奠定基础,同时为重要花香物质的分子调控机制提供理论依据。

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晏慧君
王娟
陈敏
王其刚
张颢
关键词月月粉   大马士革蔷薇   百叶蔷薇   SPME-GC/MS   香气成分   相对含量     
Abstract

Aromatic components in flower samples Rosa chinensis ‘Pallida’, R. damascene and R. centifolia were determined using the methods of headspace solid-phase microextraction(HS-SPME)and gas chromatography-mass spectroscopy (GC-MS). All sorts of volatile compounds and their relative content of petals and stamens at the opening stage were compared and analyzed, respectively. For R.chinensis ‘Pallida’, flowers mainly produce arenas and terpenes, which 1,3,5-trimethoxy-benzene is the characteristic aromatic component. There were the similar components between the R. damascene and R. centifolia, petals mainly produce alcohols, the relative content of 2-phenethyl alcohol represented was about 69% of total compounds. The different aromatic components of R. damascene and R. centifolia were elemol, methyl eugenol and geranyl acetate. The dominant volatile compound in stamens of the three rose resources was eugenol, but there is no eugenol in their petals. The results provided a foundation for cloning significant scented gene and theoretical basis for molecular regular mechanism of scent compounds in roses.

KeywordsRosa chinensis ‘Pallida’   R. damascene   R. centifolia   SPME-GC/MS   aromatic component   relative content     
Received 2015-12-21;
Fund:

国家自然科学基金项目(31360492);云南应用基础研究面上项目(2013FB093)。

Corresponding Authors: 张颢(1966-),女,云南昆明人,研究员,主要从事月季遗传育种研究。E-mail:zhanghao7898@sina.com     Email: zhanghao7898@sina.com
About author: 晏慧君(1982-),女,河南周口人,副研究员,主要从事月季花香研究。E-mail:hjyan8203@126.com
引用本文:   
晏慧君, 王娟, 陈敏, 王其刚, 张颢.月月粉(Rosa chinensis ‘Pallida’)、大马士革蔷薇(R. damascene)、百叶蔷薇(R. centifolia)香气成分分析[J]  云南农业大学学报(自然科学), 2017,V32(1): 78-82
YAN Huijun, WANG Juan, CHEN Min, WANG Qigang, ZHANG Hao.The Analysis of Scent Compounds from Rosa chinensis ‘Pallida’, R. damascene and R. centifolia by GC/MS[J]  Journal of Yunnan Agricultural University, 2017,V32(1): 78-82
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