云南农业大学学报(自然科学)
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云南农业大学学报(自然科学) 2017, Vol. 32 Issue (1) :17-26    DOI: 10.16211/j.issn.1004-390X(n).2017.01.003
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元阳梯田水稻品种‘月亮谷’根系内生及根际细菌的群落结构
赵丹1, 赵娟2, 刘涛1, 黄惠川1, 廖静静1, 靳百慧1, 朱有勇1, 何霞红1
1. 云南农业大学 植物保护学院, 云南 昆明 650201;
2. 德宏州土壤肥料工作站, 云南 芒市 678400
Study on Root Endophytic and Rhizosphere Bacteria of Rice Landrace of Yuelianggu from Yuanyang Terrace
ZHAO Dan1, ZHAO Juan2, LIU Tao1, HUANG Huichuan1, LIAO Jingjing1, JIN Baihui1, ZHU Youyong1, HE Xiahong1
1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;
2. The Soil and Fertilizer Work Station of Dehong Prefecture, Mangshi 678400, China

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摘要 

为了解元阳梯田传统水稻品种‘月亮谷’的根系内生及根际土壤的细菌群落结构,本研究以现代品种‘楚粳27’为对照,分别在云南省元阳县和建水县开展种植试验。结果显示:通过菌落分离,共得到202个菌株,结合形态特征、生理生化特性及16s rDNA对其进行初步鉴定研究,可分离到11个种和8个属;聚类分析结果表明:元阳县种植的水稻根系内生细菌的相似水平比建水县的低0.202,但其丰富度更高,差异更大;从0.890的相似水平划分,建水县种植的水稻根际土壤细菌聚为一类,较单一,表明元阳县水稻生长的微生物多样性较丰富。比较两个水稻品种在两个地点的根系内生细菌和根际土壤细菌的相对分离频率发现:假单胞菌属(Pseudomonas sp.)为优势属和共有属,恶臭假单胞菌(P.putida)是两个品种在两个地点种植的共有种,两个地点种植的‘月亮谷’,其根系和根际土壤中均能分离到荧光假单胞菌(Pseudomonas fluorescens)。研究初步表明‘月亮谷’在两个不同生态环境种植其根系内生细菌和根际土壤细菌的差异,从土壤微生态的角度阐述了‘月亮谷’能够在哈尼梯田持续种植的原因。

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赵丹
赵娟
刘涛
黄惠川
廖静静
靳百慧
朱有勇
何霞红
关键词内生细菌   根际土壤细菌   水稻传统品种   ‘月亮谷’     
Abstract

In order to explicate the community structure difference of rice root endophytic and rhizosphere bacteria in Yuanyang Terrace, ‘Yuelianggu’ and ‘Chujing 27’ were cultivated in 2 counties of Yuanyang and Jianshui. A total of 202 bacterial strains isolated were identified by morphological characteristics, physiological and biochemical analysis combined with 16s rDNA identification. The results showed that 19 categories were differentiated, including 11 species and 8 genera. Clustering analysis showed that root endophytic bacteria of rice in Yuanyang were higher diverse of 0.202 than that of Jianshui, and rhizosphere bacteria in Jianshui were less diverse than that in Yuanyang. It was suggested that micro-ecological environment of rice growth in Yuangyang was more varied when compared with Jianshui County. Pseudomonas was common and dominant bacteria on the level of genus, and Pseudomonas putida was common strain isolated from the root of 2 varieties in 2 counties, and Pseudomonas fluorescens could be always isolated from ‘Yuelianggu’ wherever it was planted in Yuanyang or Jianshui County. This study preliminarily clarified that the difference of root endophytic and rhizosphere bacteria of ‘Yuelianggu’ in two different ecosystem and it may be one of the reasons that ‘Yuelianggu’ could be continuous cultivated in Yuanyang Terrace.

Keywordsendophytic bacteria   rhizosphere soil bacteria   rice landrace   ‘Yuelianggu’     
Received 2015-12-04;
Fund:

国家重点基础研究发展计划(2011CB100400)资助。

Corresponding Authors: 何霞红(1975-),女,江西婺源人,博士,教授,主要从事农业生物多样性与病害控制研究。E-mail:hexiahong@hotmail.com     Email: hexiahong@hotmail.com
About author: 赵丹(1989-),女,云南大理人,硕士研究生,主要从事农业生物多样性与植物病害控制研究。E-mail:zd241928@163.com;赵娟(1989-),女,云南腾冲人,硕士研究生,主要从事土壤微生态研究。E-mail:zhaojuan110622@163.com
引用本文:   
赵丹, 赵娟, 刘涛, 黄惠川, 廖静静, 靳百慧, 朱有勇, 何霞红.元阳梯田水稻品种‘月亮谷’根系内生及根际细菌的群落结构[J]  云南农业大学学报(自然科学), 2017,V32(1): 17-26
ZHAO Dan, ZHAO Juan, LIU Tao, HUANG Huichuan, LIAO Jingjing, JIN Baihui, ZHU Youyong, HE Xiahong.Study on Root Endophytic and Rhizosphere Bacteria of Rice Landrace of Yuelianggu from Yuanyang Terrace[J]  Journal of Yunnan Agricultural University, 2017,V32(1): 17-26
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